DEPARTMENT OF MATHEMATICAL & STATISTICAL SCIENCES
UNIVERSITY OF ALBERTA
  

MATHEMATICAL BIOLOGY SEMINAR
 

MONDAY SEPTEMBER 9, 2002.

3:00-4:00 p.m.

CAB 657
 

Dr. Alex Mogilner

Department of Mathematics and
Center for Genetics and Development

University of California

Joint work with Dean Bottino, Tom Roberts, Murray Stewart, and George Oster.

How nematode sperm crawl

Sperm of the nematode, Ascaris suum, crawl using lamellipodial protrusion, adhesion, and retraction analogous to the amoeboid motility of other eukaryotic cells. However, rather than employing an actin cytoskeleton to generate locomotion, nematode sperm use the major sperm protein (MSP). Moreover, nematode sperm lack detectable molecular motors or the battery of actin binding proteins that characterize actin based motility. The Ascaris system provides a simple 'stripped down' version of a crawling cell in which to examine the basic mechanism of cell locomotion, independent of other cellular functions that involve the cytoskeleton. I will present a mechanochemical analysis of crawling in Ascaris sperm. We construct a finite element model wherein (a) localized filament polymerization and bundling generate the force for lamellipodial extension, and (b) energy stored in the gel formed from the filament bundles at the leading edge is subsequently used to produce the contraction that pulls the rear of the cell forward. The model reproduces the major features of crawling sperm and provides a framework in which amoeboid cell motility can be analyzed. Although the model refers primarily to the locomotion of nematode sperm, it has important implications for the mechanics of actin-based cell motility.